Hope Changes Lives
Why Patients Need You
Pfizer’s purpose is to deliver breakthroughs that change patients’ lives. Research and Development is at the heart of fulfilling Pfizer’s purpose as we work to translate advanced science and technologies into the therapies and vaccines that matter most. Whether you are in the discovery sciences, ensuring drug safety and efficacy or supporting clinical trials, you will apply cutting edge design and process development capabilities to accelerate and bring the best in class medicines to patients around the world.
What You Will Achieve
Pfizer Oncology R&D is committed to deliver breakthroughs that change patients’ lives by developing oncology agents that disrupt cancer biology or redirect the immune system against cancer cells. Integrative Biotechnologies (IBT) is a highly collaborative group that drives Pfizer’s diverse Oncology portfolio by applying expertise in chemical biology, proteomics, cell engineering, and functional genomics. We are seeking a highly self-motivated cell biologist passionate about anticancer drug discovery to join the chemical biology team of the IBT as a Senior Associate Scientist (non-PhD). Your research will focus on identifying cellular targets of small molecules, investigating drug mechanisms of action, and developing a deeper understanding of tumor biology to discover new targets. Excellent communication and organizational skills and attention to detail are essential. Experience in mammalian cell culture, protein quantification, western blotting, and immunoprecipitation is highly desirable. The successful candidate will work closely with individuals in the Medicinal Chemistry and Oncology Biology groups, learn from collaborations with cross-functional teams, and gain exposure to the broad Pfizer organization.
How You Will Achieve It
Design and execute key experiments to address small molecule target engagement and occupancy, off-target profiling, new target identification and validation studies using a variety of chemical and genetic approaches.
Culture and scale-up mammalian cells (cancer and primary cells), perform transfections and drug treatments and prepare samples for various chemical biology and proteomic studies.
Perform protein quantification, immunoprecipitation, click chemistry, and western blotting.
Develop and execute cell-based in vitro assays.
Identify and test exciting new opportunities, techniques, or strategies to help advance the drug discovery pipeline.
Document and communicate experimental findings accurately in both oral and written formats.
M.S. or B.S. with 2+ years of experience, with a degree in Biology, Biochemistry, Chemical Biology, or a related discipline.
Extensive cell culture experience with proficiency in culturing and maintaining many cell lines and/or flasks simultaneously.
Experience in preparing protein samples from purified cells, tissues, and/or FFPE, protein quantification using BCA assay, and western blotting.
Ability to design, plan, execute, and interpret experiments, and to work in a fast-paced environment- independently or as a part of a team.
Excellent organization and planning skills, good verbal and written communication, strong self-motivation and work ethic.
Familiarity with drug discovery.
Prior experience with chemical probe-based target engagement strategies, chemoproteomics, click chemistry, immunoprecipitation, and cell-based assay development.
Lifting, walking, bending, ability to perform mathematical calculations, and ability to perform complex data analysis
Perform work in a laboratory setting
Extended periods of sitting and standing
Other Job Details
Work Location Assignment: On Premise. On Premise colleagues work in a Pfizer site because it’s needed to get their job done. They may have flexibility to work remotely from time to time, but they are primarily on-site.