Senior Scientist, In vivo Gene Editing & Gene Delivery

Poseida Therapeutics

Cell and Gene Therapy Biotech

Position Summary

Poseida is seeking an outstanding candidate to join our Genetic Engineering team.  The in vivo Gene Delivery Scientist will play a key role in developing and testing our platform technologies (piggyBac transposon and Cas-CLOVER nuclease) in mouse models, working closely with the Gene Therapy and Nanotechnology groups.

 

Responsibilities

These may include but are not limited to:

·       Optimize our proprietary gene platforms by assaying new tools and/or applications for specialized and enhanced implementation in gene therapy and gene editing, specifically in mouse models, to support fundamental R&D and critical pre-clinical programs.

·       Apply your strong background working with viral vectors, transposons, molecular biology/genetics, or other gene delivery technologies to evaluate new gene delivery & editing tools in mouse models for use as in vivo therapies.

·       Work in a highly collaborative, cross-functional team environment to perform experiments in the lab and vivarium, analyze results, and communicate findings in team meetings.

·       Keep current on trends, new products and technologies in gene editing and gene delivery.

·       May manage 1-2 Research Technician or Research Associates

 

Requirements, Knowledge, Skills and Abilities

·       Ph.D. in molecular biology, genetics, cell biology, or related field with 5 plus years of hands-on experience using mouse models for in vivo genetic manipulations (an equivalent combination of education and experience may be considered).

·       Prior experience working directly with mice, including animal handling/manipulations using techniques such as hydrodynamic injections, nanoparticles, or viral vectors for nucleic acids delivery. Experience with non-viral gene delivery technology (such as transposon platforms) is strongly preferred, although not required.

·       Previous experience with functional in vivo assays used for measuring success of gene delivery and/or gene editing (e.g. luciferase or near-IR fluorescent proteins).

·       Strong background in standard molecular biology/cloning techniques/tools. Experience with BAC transgenesis and the generation of genetically engineered rodent models is a plus.

·       Demonstrated expertise in culture and genetic manipulation of primary cells and organoids, such as hepatocytes is strongly preferred, although not required.

·       Previous experience with functional in vitro assays used for measuring success of gene delivery and/or gene editing is preferred, although not required (e.g. Illumina NGS, Oxford Nanopore, LM-PCR, LAM-PCR, etc.).

·       Proficiency with multi-parameter flow cytometry, cell sorting, and FACS analysis software is a plus.

·       Must possess excellent oral and written communication skills.

 

Apply at: https://recruiting.paylocity.com/recruiting/jobs/Apply/432304/Poseida-Therapeutics-Inc/Senior-Scientist-In-vivo-Gene-Editing-Gene-Delivery

To apply for this job please visit recruiting.paylocity.com.

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